The Toxic Effect of Water-Soluble Particulate Pollutants from Biomass Burning on Alveolar Lung Cells

In 2018, 3.8 million premature deaths were attributed to exposure biomass burning nanoparticles from wood combustion. The objective of this study was investigate and compare the toxic effect wood-combustion-related three different combustion stages (i.e., flaming, smoldering, pyrolysis) on alveolar lung cells, by studying cell proliferation, structural behavioral parameters. A549 epithelial cells treated with 31, 62, 125, 250, 500 µg/mL water-soluble particulate pollutants burning, measured means real-time analysis, imaging, phase imaging microscopy. At low concentrations (31 62 µg/mL), all types samples exhibited no toxicity. 125 µg/mL, they caused decreased proliferation compared control. Exposure higher (250 µg/mL) killed cells. Cell physical parameters (area, optical volume, eccentricity, perimeter, thickness) (migration, motility, motility speed) did not change in response materials up a concentration µg/mL. changed thickness for smoldering flaming particles, led migration, speed conclusion, organic identified as equally analysis (RTCA) results. describing structure suggest that pyrolysis particles slightly less than others.